Zebrafish Heart
In collaboration with Prof Kimara Targoff, we have applied SCAPE 2.0 to imaging the zebrafish heart in 3D at over 300 volumes per second. The zebrafish embryo is a near-transparent model organism ideally suit for developmental study. Studies of the embryonic zebrafish heart can provide knowledge of the way that the vertebrate heart develops, as well as the influence of genetic and environmental factors on structural and functional cardiac development. Together with Dr. Kimara Targoff from the department of pediatrics, our studies provide new insights into the pathophysiology of congenital heart defects in humans and also enhance our understanding of therapeutic efforts to ameliorate outcomes secondary to both morphogenetic and electrophysiological abnormalities.
Together with Dr. Kimara Targoff from the department of pediatrics, our studies provide new insights into the pathophysiology of congenital heart defects in humans and also enhance our understanding of therapeutic efforts to ameliorate outcomes secondary to both morphogenetic and electrophysiological abnormalities. We employ SCAPE to image beating hearts of 3-days post-fertilization zebrafish embryos dual labelled with EGFP (endothelial cells) and DsRed (RBCs). At 321 volumes per second, individual RBCs can be tracked even inside high-speed flow areas such as the atrioventricular canal using semi-automated techniques. We also demonstrate calcium dynamics recording of beating zebrafish hearts at 100 VPS for studies of the heart’s conduction system in health and disease.
See our new SCAPE 2.0 paper for more details:
Voleti V, Patel KB, Li W, Perez Campos C, Bharadwaj S, Yu H, Ford C, Casper MJ, Yan RW, Liang W, Wen C, Kimura KD, Targoff KL, Hillman EMC. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nature Methods. 16(10):1054-62. (2019)