We utilized swept confocally aligned planar excitation (SCAPE) microscopy to measure odor-driven activity simultaneously in many (>10,000) olfactory sensory neurons distributed over large areas of intact mouse olfactory epithelium. This approach allowed us to investigate the responses to mixtures or blends of odors and their components, a more realistic stimulus than monomolecular odors. Link to press release.
Venkata Voleti's SCAPE 2.0 paper was just published in Nature Methods, and demonstrates >300 volume per second imaging in the beating embryonic zebrafish heart, along with 3D imaging of freely moving C. elegans worms and high-throughput imaging of large fixed, cleared and expanded tissues! Read our press release here.
New Preprint on BioRxiv: Glioma-induced alterations in neuronal activity and neurovascular coupling during disease progression
Diffusely infiltrating gliomas are known to cause alterations in cortical function, vascular disruption and seizures. These neurological complications present major clinical challenges, yet their underlying mechanisms and causal relationships to disease progression are poorly characterized. Here, we followed glioma progression in awake Thy1-GCaMP6f mice using in-vivo wide-field optical mapping to monitor alterations in both neuronal activity and functional hemodynamics.